Enhanced Speed and Precision With NanoWizard ULTRA Speed 3 BioAFM

The NanoWizard^® ULTRA Speed 3 BioAFM from Bruker is setting new standards when it comes to speed and performance. This instrument combines advanced automation capabilities with an unprecedented scanning speed of 1,400 lines per second. Moreover, it seamlessly integrates into sophisticated optical microscopy and super-resolution techniques.

This system provides correlated sample measurements and offers a comprehensive nanomechanical characterization of a wide array of soft matter and living biological samples. Its automated fast-scanning and analysis functionalities enhance throughput, ensuring exceptional performance.

The incorporation of novel, cutting-edge automation features introduces new opportunities for extended, self-regulating experiment series, offering unparalleled sensitivity, reliability, and precision.

Next-Level Performance

NanoWizard ULTRA Speed 3 Features a Host of New Advanced Functionalities

Internal LED lighting for stand-alone operation.

Bruker’s 3D acceleration sensor and feed-forward technology for unrivaled stability in the tip-scanning motion and the highest feedback bandwidth for excellent surface tracking.

Fully automated set-up, alignment, and re-adjustment of system parameters for easy operation and increased productivity.

DynAsyst for automatic adjustment and optimization of scan parameters in TappingMode^™.

Advanced data management and processing capabilities.

QR code reader for improved cantilever handling and management. Scans and automatically sets the parameters of Bruker’s pre-calibrated cantilevers.

NanoWizard ULTRA Speed 3 integrated into a Zeiss Axio Observer running the new Bruker SPM software version 8.1. Image Credit: Bruker Nano Analytics

TappingMode topography images of DNA origami (GATTA-AFM, Gattaquant, Germany) in TAE buffer acquired at 1,400 lines/sec. (inset). Image Credit: Bruker Nano Analytics

Morphology: The newly introduced software feature, DynAsyst, along with enhanced Dynamic PID, facilitates effortless exploration of delicate, unfixed samples at elevated speeds and minimal forces.

Dynamics: A scanning speed of 1,400 lines per second enables real-time visualization of dynamic processes.

Nanomechanics: Facilitates rapid, label-free, and multiparametric characterization of nanoscale biomechanical properties.

Microrheology: An optional fast Z-scanner featuring innovative sensor technology produces reproducible force curves at exceptional speeds. This significantly expands the frequency range for microrheological measurements.

The NanoWizard ULTRA Speed 3 sets benchmark standards by offering rapid AFM scanning capabilities that seamlessly integrate with advanced optical techniques. Its tip-scanner technology achieves scanning speeds that were previously unattainable with traditional AFMs.

Empowered Research

Nanoscale structural analysis and nanomechanical characterization deliver crucial insights into molecular, single-cell, and cellular functionality.

The NanoWizard ULTRA Speed 3 integrates rapid performance, advanced automation, and precision with user-friendly operation. This makes attaining top-quality outcomes simpler than ever, opening doors to a wide range of new applications and pioneering scientific breakthroughs.

Top row images: Left: Atomic resolution of a calcite crystal plane recorded with TappingMode AFM in water at 60 Hz line rate. 2D FFT analysis (inset) gives rectangular lattice subunit dimensions of a=0.526 nm and b=0.847 nm. Scan size: 19.5 nm × 19.5 nm. Height range: 120 pm; Center: TappingMode in liquid of puC19 DNA using Bruker probe FASTSCAN-D showing majorminor groove resolution (inset). Scan size: 87.5 nm × 87.5 nm. Height range: 3.5 nm; Right: Gasdermin pore-forming proteins imaged in lipid membrane. Overview: Scan size: 240 nm × 240 nm. Height range: 2.9 nm. Inset: Scan size: 36 nm × 36 nm. Height range: 3.5 nm. Sample courtesy Han Yu, Daniel J. Müller Lab., ETHZ, Basel, Switzerland.Bottom row images: Guided thermally driven protein kinetics on DNA nanostructures. Imaged in TappingMode at 1 frame/sec. Scan size: 150 nm × 150 nm. Height range: 6 nm. Image Credit: In collaboration with C.M. Domínguez, C.M. Niemeyer, Institute for Biological Interfaces (IBG-1), KIT, Germany.

Providing Unique Advantages

Latest dynamic PID implementation

Patented scanner technology

Seamless integration with advanced optical and super-resolution techniques

Stable scanning at speeds of up to 1,400 lines per second

Unparalleled multi-channel data acquisition rates and rapid analysis of complex, multiparametric data sets

Enhancing Capabilities

Visualizing dynamic molecular and cellular mechanisms, coupled with quantifying associated kinetics and reaction rates, enhances the understanding of fundamental biological processes. The ULTRA Speed 3, in combination with advanced optics, facilitates multiparametric observation of in-situ dynamics, providing a comprehensive approach to studying complex biological phenomena.

Single-molecule protein dynamics

Protein folding

Mechanosensitive signaling pathways

Receptor-ligand interactions

Enzymatic reactions

Kinetics of surface parameters on nanometer scale