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SorbaDex™ – For the Separation of Biomolecules
SorbaDex (also known as Cytiva Sephadex) is a beaded composite material made with ultrapure cross-linked dextran. It has good selectivity, excellent resolution, minimal non-specific adsorption, and excellent chemical stability. The effects of buffer and pH on resolution are minimal.
Superior performance, cost-effective replacement forCytiva Sephadex™
One of the major advantages of SorbaDex is its ability to quickly remove small molecules and desalt the sample into pure water simultaneously.
SorbaDex is autoclavable at 121 °C, pH 7 for 30 minutes, and is stable in all commonly used buffers.
Source: Sorbent Technologies, Inc.
| Grade | Equivalent to Cytiva Sephadex G-25 | Equivalent to Cytiva Sephadex G-50 |
|---|---|---|
| Super Fine | Dry Bead Size: 20 – 50 µm (> 80 %)Water Regain: 2.15 – 2.25 mL/gSwelling: 4 – 6 mL/gMWCO (size exclusion): below 5000 DaFractionation Range: 1 – 5 kDa (globular proteins)Catalog No.: 801001-01- 100gm | Dry Bead Size: 20 – 50 µm (> 80 %)Water Regain: 4.80 – 5.20 mL/gSwelling: 9 – 11 mL/gMWCO (size exclusion): below 25000 DaFractionation Range: 1 – 30 kDa (globular proteins)Catalog No.: 801004-01-100gm |
| Fine | Dry Bead Size: 20 – 80 µm (> 80 %)Water Regain: 2.15 – 2.25 mL/gSwelling: 4 – 6 mL/gMWCO (size exclusion): below 5000 DaFractionation Range: 1 – 5 kDa (globular proteins)Catalog No.: 801002-01 -100gm | Dry Bead Size: 20 – 80 µm (> 80 %)Water Regain: 4.80 – 5.20 mL/gSwelling: 9 – 11 mL/gMWCO (size exclusion): below 25000 DaFractionation Range: 1 – 30 kDa (globular proteins)Catalog No.: 801005-01 -100gm |
| Medium | Dry Bead Size: 50 – 150 µm (> 80%)Water Regain: 2.15 – 2.25 mL/gSwelling: 4 – 6 mL/gMWCO (size exclusion): below 5000 DaFractionation Range: 1 – 5 kDa (globular proteins)Catalog No.: 801003-01 -100gm | Dry Bead Size: 50 – 150 µm (> 80%)Water Regain: 4.80 – 5.20 mL/gSwelling: 9 – 11 mL/gMWCO (size exclusion): below 25000 DaFractionation Range: 1 – 30 kDa (globular proteins)Catalog No.: 801006-01 -100gm |
Specifications
Source: Sorbent Technologies, Inc.
| Catalog No. | Fractionation range (globular proteins) – Mr | pH stability | Bed volume ml/g dry SorbaDex™ | Bead size (dry) | Product |
|---|---|---|---|---|---|
| 801001 | 1-5 kDa | 2 to 13 | 4 – 6 | 20-50 µm | SorbaDex™ – 25 superfine |
| 801002 | 1-5 kDa | 2 to 13 | 4 – 6 | 20-80 µm | SorbaDex™ – 25 fine |
| 801003 | 1-5 kDa | 2 to 13 | 4 – 6 | 50-150 µm | SorbaDex™ – 25 medium |
| 801004 | 1-30 kDa | 2 to 13 | 9 – 11 | 20-50 µm | SorbaDex™ – 50 superfine |
| 801005 | 1-30 kDa | 2 to 13 | 9 – 11 | 20-80 µm | SorbaDex™ – 50 fine |
| 801006 | 1-30 kDa | 2 to 13 | 9 – 11 | 50-150 µm | SorbaDex™ – 50 medium |
| 801007 | Hydrated in phosphate buffered saline; pH 7.4, with 0.2% sodium azide | SorbaDex™ – 50 fine | |||
| 801008 | SorbaDex™ – 25 medium | ||||
Performance
Source: Sorbent Technologies, Inc.
| . | . |
|---|---|
|
High Performance Results |
 |
| Protein Desalting from 0.8M NaClIgG (280 nm): dark blue lineNaCl (µS): gold line1 mg IgG anti-Rabbit in 1 mL 0.8 M sodium chlorideWater elution |  |
| Removal of FITC from IgGIgG (280 nm): dark blue lineFITC (550 nm): gold line1 mg IgG anti-Rabbit and 0.1 µmol FITC in 1 mL DMSO/NaHCO3PBS Elution |  |
| Oligo Desalting from 0.8M NaClOligo (260 nm): dark blue lineNaCl (µS): gold line1 mg oligonucleotide (18-mer) in 1 mL 0.8 M NaCl |   |
| Removal of Rhodamine after labelingOligo (260 nm): dark blue lineTAMRA (550 nm): gold line1 mg oligonucleotide (18-mer) and 0.5 µmol TAMRA in 1 mL DMSO/NaHCO3 |  |
| Removal of Ammonia (33%)Dextran Blue (260n m): dark blue lineNH3(pH): gold line0.5 mg Dextran Blue (Mr 2,000,000) in 1 mL ammonia (33%)Water elution |  |
| Removal of Ammonia (33%) from OligoOligo (260 nm): dark blue lineNH3(pH): gold line1 mg oligonucleotide (18-mer) in 1 mL ammonia (33%) after cleavage |  |
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