Optilab Refractive Index Detector for HPLC/GPC

上海仪舶实验室自动化 25-01-23 13:00:26

Optilab Refractive Index Detector for HPLC/GPC

The Optilab® differential refractive index (dRI) detector may be used in conjunction with any HPLC, FPLC, GPC or FFF separation system, plus a Wyatt DAWN® or miniDAWN® multi-angle light scattering (MALS) detector, for the absolute determination of the molar mass of all types of macromolecules in solution. While its sensitivity matches the very best HPLC RI detectors, its dynamic range exceeds that of any comparable instrument by 10-100x, with no range settings - meaning you can characterize very large and very small peaks, simultaneously.

In addition to dRI, the Optilab also measures the absolute refractive index (aRI) of a solution, important for characterizing solvents. The high-concentration (HC) model provides an extended measurement range suitable for high-concentration samples (up to 180 mg/mL of protein) or gradient-elution chromatography such as ion-exchange.

Features

dRI noise: 7.5x10-10 RIU (HC model: 1.5x10-9 RIU)

dRI range: ±4.7x10-3 RIU (HC model: -2.6x10-3 to +34x10-3 RIU)

Dynamic range: 12,000,000:1 (HC model: 23,000,000:1)

Absolute RI sensitivity: ±0.002 RIU

Absolute RI range: 1.2 – 1.8 RIU

Temperature control: heated/cooled 4 - 65°C, including active stabilization—even at room temperature

Wavelength: 660 nm, matched to MALS instruments; other wavelengths available

Touch-panel display for immediate access to data, diagnostics and settings

Applications

Universal online concentration measurement for chromatography, especially SEC-MALS and FFF-MALS.

Analysis of conjugated proteins, co-polymers and nanoparticle-encapsulated drugs/proteins/DNA/RNA when combined with a MALS and UV detector

Offline measurement of second virial coefficient A2 (B22)

Offline measurement of the specific refractive index increment dn/dc, at the same wavelength as the MALS laser (critical for accurate macromolecular characterization)

Ion-exchange chromatography with MALS (IEX-MALS) – measure dRI over the entire salt gradient

Composition-gradient MALS (CG-MALS) – measure protein-protein interactions at high concentration

[Optilab Refractive Index Detector for HPLC/GPC]相关推荐
今日仪器
  1. Archway Engineering (UK) Ltd

    Archway Engineering (UK) Ltd

    Archway Engineering is one of the UK’s leading manufacturers of drilling equipment for site investigation, mineral exploration, water well construction, and environmental monitoring. We manuf……

    代理品牌 2025-07-31

  2. HMK-CD5 Laser Particle Size Analyzer

    HMK-CD5 Laser Particle Size Analyzer

    The HMK-CD5 laser particle size analyzer is a new generation of analyzers developed by Aimsizer Scientific. It is made by ISO 13320 and ISO 13321 and operates on the two most advanced theories. Pho……

    仪器产品 2025-07-31

  3. Vacuum Processes, Inc.

    Vacuum Processes, Inc.

    VPI is a complete turnkey design, manufacturing, service, engineering, research, and installation entity, which provides custom vacuum and thermal process equipment for various industries. Our miss……

    代理品牌 2025-07-31

返回顶部小火箭