NanoRacer High-Speed AFM

The NanoRacer^® High-Speed AFM denotes a quantum leap in quantitative imaging abilities. The dynamic biological processes’ real-time visualization of nanometer resolution is simple.

The NanoRacer paves the way for a world of new and stimulating chances for Life Science applications. This allows scientists to gain in-depth knowledge of complicated biological systems and molecular mechanisms, in a way that has not been possible so far.

Benefits

50 fps and 5000 lines/second

Small cantilever capability is available for the lowest force imaging and least sample damage.

Highest scan speeds for progressed force mapping

Atomic defect resolution

Real-time visualization of dynamic biological processeswith nm resolution.

Comprehending complicated molecular dynamics and biological systems.

Automation with fast results

Outstanding performance, cutting-edge engineering, and stability.

Users can completely perform the automated setup.

Advanced data analysis.

Features

NanoRacer offersa new chapter in high-speed atomic force microscopy (AFM), providing an in-depth understanding ofmolecular dynamics in real-time at 50 frames and true 5000 lines per second.

The NanoRacer unveils a world of new and interesting chances for Life Science applications. This allows scientists to gain an elaborate insight into complicated biological systems and molecular mechanisms, in a method that is notpossible so far.

Features include:

Single-molecule binding behavior

Enzyme activity monitoring

Protein/protein interactions

Virus and bacteria morphology and dynamics

Availability of motor protein and membrane trafficking dynamics

Dynamics available in two-dimensional protein assemblies

Assembly and disassembly processes of protein structures

DNA origami assembly

DNA origami nanostructures containing 5 biotin binding sites on mica, imaged in fluid with streptavidin presence in closed-loop at 50 frames and 5000 lines/sec. Image Credit: Bruker Nano GmbH - JPK BioAFM

So many things are still hidden in biological molecules. To uncover their unexplored secrets, there is a true need to directly observe individual molecules during their functional activity. The NanoRacer is the fastest commercial, high-speed AFM available, enabling their direct observation in real-time.

Toshio Ando, Professor, Nano Life Science Institute (WPI-NanoLSI), Kanazawa University

Ando stated, “A lot of innovative ideas have been incorporated for easy operation and high performance, and it is my utmost wish that many researchers will use the NanoRacer to reach their goals and make exciting discoveries.”

Image Credit: Bruker Nano GmbH - JPK BioAFM

Outstanding Resolution, Renowned Stability, Remarkable Accuracy

Imaging atomic defects and sub-molecular resolution are routine. The NanoRacer consists of the least noise levels of any commercial AFM system available due to the high-precision electronics and improved precision positioning sensors present in every axis.

The NanoRacer shows the pioneering work of Bruker’s BioAFM team in integrating technological progress with user-friendliness, proven stability, and sensitivity.

Atomic resolution of calcite crystal step edge, imaged in fluid, 3D topography 15 × 9 nm² [1], zoom 4 × 4 nm² [2]. Image Credit: Bruker Nano GmbH - JPK BioAFM

Availability of small cantilevers and lowest forces for minimum sample damage

Advanced algorithms can be performed for scanner control and feedback

Minimum force drift available for long-term experiments

Cutting-edge, high-speed power amplifier provided for ideal scanner drive

Closed-loop scanning on all axes with minimal noise levels for the greatest precision

Infrared laser photothermal excitation optionfor clean cantilever drive, simple setup, and reduced perturbance of sensitive samples

Greatest bandwidth digital electronics, with the lowest noise, for utmost performance

Individual DNA molecules imaged in fluid on mica+PLO in closed loop. Sequences [4] + [5] are imaged at 50 frames/sec. Click on the image to watch the video. Image Credit: Bruker Nano GmbH - JPK BioAFM

Discover a New User Experience - A Complete System Designed for Convenience

Simple sample and probe loading

Transportable sample carrier for comfortable sample preparation on the bench

Fluid exchange through direct injection

Newly developed three-port liquid cell available for photothermal excitation

No calibration is required as a result of the closed-loop scanner design

Simple navigation can be carried out with a combined camera for locating areas of interest on the sample

Probe exchange can be done in just a few minutes

Image Credit: Bruker Nano GmbH - JPK BioAFM

The NanoRacer provides anew chapter in high-speed AFM and relegates complex, laborious procedures to the past. Produced with the user in mind, thestrong and reliable design, with a host of new features, makes the NanoRacer user-friendly, even for newcomers to AFM.

All components have been developed for comfortable handling, from sample preparation to completely motorized and automatic optical alignment. The smooth handling enables simple data collection and quick outcomes. A brief time-to-data is crucial for obtaining dynamic outcomes on active single-molecule samples.

Improved drift compensation

Automated cantilever alignment can be performed

Automated calibration of cantilever spring constant

Availability of internal camera with autofocus

Automated photothermal laser alignment option is available

Seamless handling for preparation and imaging with the transportable sample scanner. Prepare the sample conveniently on the bench and load into the NanoRacer to image. Image Credit: Bruker Nano GmbH - JPK BioAFM

Key Features

Greatest imaging speed of 50 frames and true 5000 lines per second with excellent resolution

Availability of intuitive V7 software for proven user-friendliness

Newly developed scanner unit and high-speed head

Improved for small and medium-sized cantilevers

Cutting-edge electronics available

Strong concentric design for maximum stability

Optional with Bruker’s exclusive PeakForce Tapping

Automated cantilever alignment is possible

Image Credit: Bruker Nano GmbH - JPK BioAFM

Applications

High-Speed Imaging Data Gallery

Bruker’s BioAFMs enable life science and biophysics scientists to advance their investigations in mechanobiology, cell dynamics, cell mechanics and adhesion, cell-cell and cell-surface interactions, and cell morphology.