FLUOstar Omega Filter-Based Multi-Mode Microplate Reader

Detection modes

Fluorescence intensity - incl. FRET

AlphaScreen^®/AlphaLISA^®

Luminescence (flash and glow) - incl. BRET

Time-resolved fluorescence

TR-FRET

UV/Vis absorbance spectra

Measurement modes

Top and bottom reading

Endpoint and kinetic

Sequential multi excitation

Sequential multi emission

Spectral scanning (absorbance)

Ratiometric measurements

Well scanning

Microplate formats

6 to 384-well plates, user-definableLVis Plate with 16 low volume microspots (2 μL)

Microplate carrier

Robot compatible

Light sources

High energy xenon flash lamp

Detectors

Low noise photomultiplier tubeCCD spectrometer

Wavelength selection

Optical filters: ex and em wheels for 8 filters eachUV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 s/well

Optical filters

Excitation and emission filter wheels for 8 filters each

Optical path guides

Top: liquid-filled light guidesBottom: fiber optics

Spectral range

Filters: 240–740 nm or 240–900 nm for FI, FP, TRF240–740 nm for LUM

Spectrometer: 220–1000 nm for ABS

Sensitivity

Read times

Flying mode (1 flash), 9 s (96), 16 s (384)

Reagent injection

Up to 2 built-in reagent injectorsInjection at measurement position (6 to 384-well)Individual injection volumes for each well (3 to 500 µL)Variable injection speed up to 420 µL/sUp to four injection events per wellReagent back flushing

Shaking

Linear, orbital, and double-orbital with user-definable time and speed

Purge gas vent

System to inject an atmosphere or to pull a vacuum into the reader

Incubation

+4 °C above ambient up to 45 °C or 65 °CThe upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Software

Multi-user Reader Control and MARS Data Analysis Software includedFDA 21 CFR Part 11 compliant

Dimensions

Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg

LVis plate

Sample capacity: 16 separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.

Quality control internal standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates.

Stacker

Magazines for up to 50 plates - continuous loading feature

THERMOstar

Microplate incubator and shaker

Optical filters

Excitation and emission filters

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.

LOD = 3x SD (20 blanks)/slope (6 pt std curve)AlphaScreen^® P-Tyr-100 assay kit, PerkinElmer, #6760620CMicroplates: white for LUM, AlphaScreen^®, TRF; black for FI, FP; clear for ABS96 = 96-well microplates384 sv = 384-well small volume microplates384 g = 384-well glass bottom microplates