B&P Littleford
B&P Littleford designs and builds a wide spectrum ofmixing, drying, compounding, reacting, ex……...
The FLUOstar® Omega is an ideal plate reader for all life science applications. It is a multi-mode microplate reader, with six detection modes. For absorbance, it uses an ultra-fast UV/vis spectrometer or filters, and highly sensitive filters for all other detection modes.
Product Details
The six detection modes used in the versatile FLUOstar® Omega multi-mode plate reader are:fluorescence intensity (including FRET), luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®.
Features
High-speed absorbance measurements
Measurements in up to 384-well plates
Top and bottom reading
Incubation up to 45 or 65 °C
User-defined shaking
Well scanning with up to 900 data points per well
High-precision injectors with simultaneous reagent injection and detection
Low-volume measurements
Accessories
Atmospheric Control Unit (ACU): provides the ideal environment for cell-based assays by regulating O2 and CO2 gas levels in the microplate reader
Filters and Optic Modules: standard filters from 240 - 850 nm, filters and Optic Modules optimised for specific applications, and bespoke solutions are available
Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purposes
Technical Specifications
Detection modes |
Fluorescence intensity - incl. FRET AlphaScreen®/AlphaLISA® Luminescence (flash and glow) - incl. BRET Time-resolved fluorescence TR-FRET UV/Vis absorbance spectra |
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Measurement modes |
Top and bottom reading Endpoint and kinetic Sequential multi excitation Sequential multi emission Spectral scanning (absorbance) Ratiometric measurements Well scanning |
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Microplate formats |
6 to 384-well plates, user-definableLVis Plate with 16 low volume microspots (2 μL) |
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Microplate carrier |
Robot compatible |
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Light sources |
High energy xenon flash lamp |
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Detectors |
Low noise photomultiplier tubeCCD spectrometer |
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Wavelength selection |
Optical filters: ex and em wheels for 8 filters eachUV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 s/well |
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Optical filters |
Excitation and emission filter wheels for 8 filters each |
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Optical path guides |
Top: liquid-filled light guidesBottom: fiber optics |
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Spectral range |
Filters: 240–740 nm or 240–900 nm for FI, FP, TRF240–740 nm for LUM Spectrometer: 220–1000 nm for ABS |
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Sensitivity |
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Read times |
Flying mode (1 flash), 9 s (96), 16 s (384) |
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Reagent injection |
Up to 2 built-in reagent injectorsInjection at measurement position (6 to 384-well)Individual injection volumes for each well (3 to 500 µL)Variable injection speed up to 420 µL/sUp to four injection events per wellReagent back flushing |
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Shaking |
Linear, orbital, and double-orbital with user-definable time and speed |
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Purge gas vent |
System to inject an atmosphere or to pull a vacuum into the reader |
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Incubation |
+4 °C above ambient up to 45 °C or 65 °CThe upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer. |
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Software |
Multi-user Reader Control and MARS Data Analysis Software includedFDA 21 CFR Part 11 compliant |
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Dimensions |
Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg |
Optional Accessories
LVis plate |
Sample capacity: 16 separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples. Quality control internal standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy Dimensions: conforms to SBS standards for microplates. |
Stacker |
Magazines for up to 50 plates - continuous loading feature |
THERMOstar |
Microplate incubator and shaker |
Optical filters |
Excitation and emission filters |
Upgrades |
Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc. |
LOD = 3x SD (20 blanks)/slope (6 pt std curve)AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620CMicroplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS96 = 96-well microplates384 sv = 384-well small volume microplates384 g = 384-well glass bottom microplates |
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