DynaPro ZetaStar Light Scattering Detector

The DynaPro Zetastar is an instrument that combines measurements of dynamic and static light scattering (DLS/SLS) or dynamic and electrophoretic light scattering (DLS/ELS) simultaneously.

Microcuvette measurements can be carried out manually or automatically using a flow cell and an autosampler. This can swiftly evaluate parameters for characterization and stability investigations, including size, polydispersity, zeta potential, charge, particle concentration, molar mass, or turbidity.

Key Features

DYNAMICS Touch on-board application for self-directed, walk-up operation

With as little as 2 µL of solution, users can assess size and turbidity, or zeta potential with 65 µL of solution

Adaptive collection for zeta potential measurements that are quicker and easier

In buffers with conductivity up to two times physiological saline, measure the zeta potential

A variety of high-quality disposable cells, cuvettes, and quartz cells for zeta potential and size measurements

Specifications

Source:Waters | Wyatt Technology

test	test
Pressure Range	Flow cell pressurizable to 400 psi for bubble suppression in conductive solvents
Optical System	Multiple optical channels: in-cuvette DLS & SLS, in-cuvette or flow cell DLS & ELS, fiber connection to Wyatt MALS detector for online DLS.ELS optical system: fiber-coupled interferometric Doppler electrophoretic light scattering (FIDELIS), determines electrophoretic mobility and zeta potential from frequency shift of scattered light
Refractive Index Range	N/A
Noise Level	N/A
Plate Formats	N/A
Measurement Mode(s)	ELS: electrophoretic mobility, zeta potential, electrical chargeDLS: size, size distributions, polydispersitySLS: particle concentration, molar mass, turbidity
Applications	Analysis of proteins, gene vectors, viruses and virus-like particles, promiscuous inhibitors, micelles, nano-emulsions, lipid and metallic nanoparticles, and more.
Linearity	SLS detector is linear over 6 decades
Principle	Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS)
Sensitivity	DLS: 0.1 mg/mL of lyozymeELS: 1 mg/mL BSASLS: 0.1 mg/mL lysozyme
Measuring Range	Size: 0.2 nm to 1000 nm (hydrodynamic radius)Zeta potential: No practical limitMolecular weight: 300 Da to 1 MDa
Wavelength(s)	785 nm
Type	Dynamic Light Scattering (DLS), Electrophoretic Light Scattering (ELS), Static Light Scattering (SLS)
Stability	N/A
Measuring Time	15 seconds typical
Radius of Gyration Range	N/A
Detection System	DLS: Avalanche photodiode at 90º or 163.5º scattering angleSLS: photodiode at 90º scattering angleELS Detection: photodiode at 10º scattering angle for FIDELIS (fiber-interferometric Doppler electrophoretic light scattering)
Data Acquisition Rate	N/A
Sample Volume	Quartz Cuvette: 2 µL and 45 µLPEEK Flow Cell: 350 µLDip Cell: 65 µLDisposable DLS Cuvette: 4 µLDisposable Zeta Cell: 400 µL
Max. Flow Rate	N/A
Dimensions (WxDxH)	36 x 57 x 20 cm
Shear Rate Range	N/A
Max. Pressure	N/A
Detector(s)	N/A
Temperature Range	-10 °C to +120 °C
Interface	DYNAMICS TOUCH onboard app or DYNAMICS Software on PC
Sample Type	Proteins, polymers, and nanoparticles including viruses, liposomes, gene vectors, nanoemulsions, etc.
Cell Volume	N/A
Control	Software control of detector and autosampler system to automate a series of unattended measurements
Particle Size	0.2 to 1000 nm
Light Source	785 nm laser, programmable 10% to 100%
Dynamic Range	N/A

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