DynaPro Plate Reader Dynamic and Static Light Scattering Detector

The DynaPro^® Plate Reader performs automated analysis of the size and molar mass of peptides, proteins, polymers, nanoparticles and emulsions using dynamic and static light scattering (DLS and SLS) in microwell plates.

All measurements take place in situ in microwell plates with no additional liquid handling, and samples are fully recoverable. The DynaPro enables novel, high-throughput workflows that simply would not be possible with typical cuvette-based DLS instruments.

Features

Uses industry-standard, flat-bottom 96, 384 or 1536-well plates

DLS Sensitivity: 0.125 µg of 14.4 kDa lysozyme

Hydrodynamic radius (R_h) range: 0.5 – 1000 nm

SLS sensitivity: 1 mg/mL of 66.7 kDa BSA

Molar mass range: 1 kDa – 1 MDa

Minimum sample volume: 4 µL in 1536-well plates

Temperature control: 4 – 85 °C

Typical scan time for a fully-loaded 386-well plate: 90-120 minutes

Infrared laser wavelength of 830 nm, not susceptible to fluorescence

Embedded camera images the bottom of each well to identify precipitation, turbidity and bubbles

Touch-panel display for immediate access to data, diagnostics and settings

Software: DYNAMICS^® analysis software offers easy Experiment Builder method setup; comprehensive analysis of size and size distributions, molar mass, diffusion interaction parameter k_D, second virial coefficient A2, thermal behavior T_agg/T_m/T_onset, DLS-based viscosity; versatile heatmap display of results; full export of all results; optional 21CFR11 support for GMP

Applications

Screen biotherapeutic candidates and formulations for developability and stability.

Protein quality control before interaction screening

Optimize protein buffers for crystallization and purification

Identify ideal dissolution and processing conditions for nanoparticle or polysaccharide production

Critical micelle concentration and temperature of surfactants or polymers