DynaPro NanoStar II Dynamic Light Scattering Detector

The DynaPro^® NanoStar^®II is a cuvette-based dynamic light scattering (DLS) instrument used for the analysis of the size and size distributions of peptides, proteins, polymers, nanoparticles and emulsions. A separate, optimized static light scattering (SLS) channel offers true molar mass measurements.

The NanoStar IIis among the most sensitive, robust and reliable DLS detectors available for protein and nanoparticle analysis.

Features

Performance

Sensitivity: 0.1 mg/mL of 14.4 kDa lysozyme in PBS

Molar mass range: 300 Da to 1 MDa

Particle Concentration Range: 1x10⁵to 1x10¹⁵mL^-1(dependent on size)

Size RangeR_hby DLS: 0.2 to 1000 nm

Temperature Range

-10°C to +120°C (0°C to 80°C with disposable cuvettes)

Maximum ramp rate 15°C/min

Stability: ± 0.01°C

Sample Options - Sample Cells

Quartz 2µL or 45µL cuvettes

Disposable 4µL cuvette (cyclic olefin copolymer)

Generic 4 mL quartz and disposable cuvettes (with adapter)

Sample Options - Minimum Sample Volume

2µL in quartz cuvette

4µL in disposable cuvette

Optics

Laser Wavelength: 658 nm

Laser Power Control: Programmable 10% to 100%

Scattering Angle: 90º

Optical Fiber: Multi-mode

Additional Detectors: Laser monitor for active stabilization

Additional Detectors:Forward monitor measures transmitted light for turbidance and laser/cell diagnostics

Electronics

Data Acquisistion Time: 1 to 3600 sec

Correlator: 512 channel, 100 nsec sampling time in a multi-tau layout

Dimensions:

57 cm (L) x 36 cm (W) x 20 cm (H)

Applications

Size, aggregation and stability of small molecules, peptides, proteins, vesicles, nanoparticles, quantum dots and low/sub-micron colloids

Protein quality control for interaction or structural studies

Protein and polymer weight-average molecular weight

Macromolecular interactions: diffusion interaction parameter k_D and second virial coefficient A₂ (B₂₂)

Thermal onset parameters T_agg, T_onset, T_m

Solubility of polymers

Critical micelle concentration and temperature

Nanoparticle structure by combining MALS and DLS sizing